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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
15/06/2021 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SIMÕES, L. M. S.; SANTOS, A. P. C.; BOTTINO, M. P.; LIMA, E. A.; FERNANDES, U. R.; ORLANDI, R. E.; RODRIGUES, S. A. D.; CAIXETA, F. M.; ALVES, N. G.; SOUZA, J. C.; QUINTAO, C. C. R.; CAMARGO, L. S. A.; DODE, M. A. N.; SALES, J. N. S. |
Afiliação: |
L. M. S. SIMÕES, UFLA; A. P. C. SANTOS, UFLA; M. P. BOTTINO, UFLA; E. A. LIMA, UFLA; U. R. FERNANDES, UFLA; R. E. ORLANDI, UFLA; S. A. D. RODRIGUES, UNB; F. M. CAIXETA, UNB; N. G. ALVES, UFLA; J. C. SOUZA, UFLA; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; MARGOT ALVES NUNES DODE, Cenargen; J. N. S. SALES, UFLA. |
Título: |
Nuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Theriogenology, v. 166, p. 90-96, 2021. |
DOI: |
https://doi.org/10.1016/j.theriogenology.2020.12.021 |
Idioma: |
Inglês |
Conteúdo: |
The maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability. MenosThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of met... Mostrar Tudo |
Palavras-Chave: |
Fertilização in-vitro; IFOT; Intra-follicular. |
Thesagro: |
Bovino; Fertilidade Animal. |
Thesaurus Nal: |
Embryo (animal). |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03144naa a2200361 a 4500 001 2133325 005 2021-09-15 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.theriogenology.2020.12.021$2DOI 100 1 $aSIMÕES, L. M. S. 245 $aNuclear maturation kinetics and in vitro fertilization of immature bovine oocytes injected into pre-ovulatory follicles.$h[electronic resource] 260 $c2021 520 $aThe maturation kinetics and in vitro fertilization of immature bovine oocytes injected by the intrafollicular oocyte injection (IFOT) technique into pre-ovulatory follicles of previously synchronized cows were evaluated. In Experiment 1, grade I, II and III cumulus-oocyte complexes (COCs) were randomly distributed to one of three Groups: Matvitro22 (COCs matured in vitro for 22 h), MatFol20 and MatFol28 (COCs matured in vivo after being injected into a pre-ovulatory follicle of previously synchronized cows for 19.8 ± 0.1 h and 28.3 ± 0.1 h, respectively). Cows received 12.5 mg of LH (Lutropin, Bioniche, Canada) at the time of IFOT in the MatFol20 Group or 10 h after IFOT in the MatFol28 Group. MatFol20 and MatFol28 COCs were aspirated approximately 20 h after the LH injection for nuclear maturation kinetics and recovery rate assessment. In Experiment 2, grade I, II, and III COCs were randomly distributed into two Groups: Matvitro22 Group, COCs were matured and fertilized in vitro, and MatFol20 Group, COCs were matured as in the MatFol20 Group in Experiment 1, but COCs were fertilized in vitro. Putative zygotes were classified as fertilized, unfertilized or polyspermic. In Experiment 1, the recovery rate was lower (P < 0.001) in the MatFol20 Group (52.9%, 91/172) compared with MatFol28 (72.9%, 113/155). Rate of oocytes in germinal vesicle stage, metaphase I, anaphase I and telophase I were similar among Groups. However, oocytes matured in vivo for 28.3 h had lower rate of metaphase II (P ¼ 0.001) and greater rates of degenerated (P ¼ 0.001) and parthenogenetically activated (P ¼ 0.001) oocytes. In experiment 2, the rates of polyspermy and degenerated were similar between Groups. However, the rate of fertilized oocytes was greater (P ¼ 0.05) in oocytes in the MatFol20 Group. It is concluded that oocyte in vivo maturation for 19.8 h after IFOT does not compromise the nuclear maturation kinetics and increases in vitro fertilization rates. However, the extra 10 h of intra-follicular incubation time decreased oocyte viability. 650 $aEmbryo (animal) 650 $aBovino 650 $aFertilidade Animal 653 $aFertilização in-vitro 653 $aIFOT 653 $aIntra-follicular 700 1 $aSANTOS, A. P. C. 700 1 $aBOTTINO, M. P. 700 1 $aLIMA, E. A. 700 1 $aFERNANDES, U. R. 700 1 $aORLANDI, R. E. 700 1 $aRODRIGUES, S. A. D. 700 1 $aCAIXETA, F. M. 700 1 $aALVES, N. G. 700 1 $aSOUZA, J. C. 700 1 $aQUINTAO, C. C. R. 700 1 $aCAMARGO, L. S. A. 700 1 $aDODE, M. A. N. 700 1 $aSALES, J. N. S. 773 $tTheriogenology$gv. 166, p. 90-96, 2021.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
03/01/2024 |
Data da última atualização: |
03/01/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
PEREIRA, L. L.; CARDOSO, W. S.; GUARÇONI, R. C.; FONSECA, A. F. A. da; MOREIRA, T. R.; CATEN, C. S. T. |
Afiliação: |
LUCAS LOUZADA PEREIRA, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; WILTON SOARES CARDOSO, INSTITUTO FEDERAL DO ESPÍRITO SANTO; ROGÉRIO CARVALHO GUARÇONI, INSTITUTO CAPIXABA DE ASSISTÊNCIA TÉCNICA, PESQUISA E EXTENSÃO; AYMBIRE FRANCISCO A DA FONSECA, CNPCa; TAÍS RIZZO MOREIRA, UNIVERSIDADE FEDERAL DO ESPÍRITO SANTO; CARLA SCHWENGBER TEN CATEN, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL. |
Título: |
The consistency in the sensory analysis of coffees using Q-graders. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
European Food Research and Technology, Berlin, v.243, n. 9, p.1545-1554, 2017. |
Idioma: |
Inglês |
Conteúdo: |
The process of sensory evaluation is widely used in the coffee classification worldwide. This evaluation is popularly known as “Cupping Test” and constitutes the principal methodology to assess the final quality of the coffee drink. This study observed the effect of the interaction among Q-graders, as well as the coffee tasting process in the morning and in the afternoon. The study was conducted with trained tasters with Q-Grader certificates. The methodology followed the analysis protocol guidelines of the Specialty Coffee Association of America, SCAA, with the participation of two testing groups, each one with two Q-graders for the analysis. T Tests, followed by the Pearson’s correlation and the analysis of hierarchical grouping were used for the data analysis. The results indicate that the tasters have full capacity of evaluation, although there are variances in relation to the perceptions of the attributes that define the best coffees. The results also indicate that the effect of the shift, when isolated from interaction, interfere neither in the evaluation of the batches, nor the performance of the tasters; however, when associated with interaction (chatting), the analysis indicate distortions, highlighting the need of improving the techniques of sensory evaluation. |
Thesagro: |
Coffea Arábica. |
Thesaurus NAL: |
Descriptive studies; Statistics. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01949naa a2200217 a 4500 001 2160428 005 2024-01-03 008 2017 bl uuuu u00u1 u #d 100 1 $aPEREIRA, L. L. 245 $aThe consistency in the sensory analysis of coffees using Q-graders.$h[electronic resource] 260 $c2017 520 $aThe process of sensory evaluation is widely used in the coffee classification worldwide. This evaluation is popularly known as “Cupping Test” and constitutes the principal methodology to assess the final quality of the coffee drink. This study observed the effect of the interaction among Q-graders, as well as the coffee tasting process in the morning and in the afternoon. The study was conducted with trained tasters with Q-Grader certificates. The methodology followed the analysis protocol guidelines of the Specialty Coffee Association of America, SCAA, with the participation of two testing groups, each one with two Q-graders for the analysis. T Tests, followed by the Pearson’s correlation and the analysis of hierarchical grouping were used for the data analysis. The results indicate that the tasters have full capacity of evaluation, although there are variances in relation to the perceptions of the attributes that define the best coffees. The results also indicate that the effect of the shift, when isolated from interaction, interfere neither in the evaluation of the batches, nor the performance of the tasters; however, when associated with interaction (chatting), the analysis indicate distortions, highlighting the need of improving the techniques of sensory evaluation. 650 $aDescriptive studies 650 $aStatistics 650 $aCoffea Arábica 700 1 $aCARDOSO, W. S. 700 1 $aGUARÇONI, R. C. 700 1 $aFONSECA, A. F. A. da 700 1 $aMOREIRA, T. R. 700 1 $aCATEN, C. S. T. 773 $tEuropean Food Research and Technology, Berlin$gv.243, n. 9, p.1545-1554, 2017.
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Embrapa Café (CNPCa) |
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Biblioteca(s): |
Catálogo Coletivo de Periódicos Embrapa; Embrapa Acre; Embrapa Algodão; Embrapa Amazônia Oriental; Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Gado de Corte; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Semiárido; Embrapa Soja; Embrapa Suínos e Aves; Embrapa Trigo. MenosCatálogo Coletivo de Periódicos Embrapa; Embrapa Acre; Embrapa Algodão; Embrapa Amazônia Oriental; Embrapa Arroz e Feijão; Embrapa Cerrados; Embrapa Gado de Corte; Embrapa Meio Norte / UEP-Parnaíba; Embrapa Semiárido; Embrapa Soja... Mostrar Todas |
Identificador: |
2431 |
Data corrente: |
09/05/2002 |
Data da última atualização: |
09/05/2002 |
Código do título: |
5150982 |
ISSN: |
0010-065X |
Código CCN: |
023259-9 |
Título e Subtítulo: |
COLEOPTERISTS BULLETIN |
Entidade: |
Coleopterists Society |
Local de publicação: |
Chicago-IL |
Periodicidade: |
trimestral |
Inicio de publicação: |
1947 |
Coleções da unidade: |
Embrapa Acre 1977 31 (3-4); 1993 47 (3); 1995 49 (1-4); 1997 51 (1-4); 1998 52 (1-4); 1996 50 (1-4) Classificação: 595.7605
Embrapa Algodão 1958/93 12(1); 13-20; 21(1-2,4); 22; 23(1-2); 24(1,3-4); 25(2-4); 26(2-4); 27-29; 31-39; 46(1-2,4); 47(1-2); 55(1)2001; 56(supl.)2002. Classificação: 595.760 5
Embrapa Amazônia Oriental 1977 31(1-4); 1978 32(1-4); 1979 33(1-4); 1980 34(1-4); 1981 35(1-4); 1982 36(1-4); 1983 37(1-4); 1984 38(1-4); 1985 39(1-4); 1986 40(1-4); 1987 41(1-4); 1989 43(1-4); 1990 44(1-4); 1992 46(1-4); 1993 47(1-4) Classificação: 595.7605C1
Embrapa Arroz e Feijão 1977 31(1-4); 1978 32(1-4); 1979 33(1-4); 1980 34(1-4); 1981 35(1-4); 1982 36(1-4); 1983 37(1-4); 1984 38(1-4); 1985 39(1-4)
Embrapa Cerrados 1977-87 31-41; 1989-90 43-44; 1997 51(1-2); 1999 53(4); 2000 54(1-4); 2001 55 (1-4); 2002 56 (1-4) Classificação: 595.7605
Embrapa Gado de Corte 1977-79 31-33; 1980 34(1); 1984 38(1,4); 1991 45; 1992 46(3); 1996-98 50-52; Classificação: 595.7005
Embrapa Meio Norte / UEP-Parnaíba 1977-79 31-33(1-4); 1980 34(1) Classificação: 595.7605
Embrapa Semiárido 1977 31(1-4); 1978 32(1-4); 1979 33(1-4); 1980 34(1-4); 1981 35(1-4); 1982 36(1-4); 1983 37(1-4); 1984 38(1-4); 1985 39(1-4); 1986 40(1-4); 1987 41(1-4); 1989 43(1-4); 1990 44(1-4); 1992 46(1-4); 1993 47(1-4); 1994 48(1-4); 1995 49(1-4); 1997 51(4);
Embrapa Soja 1977 31; 1978 32(2-4); 1979 33(3-4); 1980-86 34-40 Classificação: 595.7605
Embrapa Suínos e Aves 1977-87 31-41(1-4); 1989 43(1-4) Classificação: 595.7605
Embrapa Trigo 1977/85 31 1977; 32; 1978; 33 1979; 34 1980; 35 1981; 36 1982; 37 1983; 38(1-3) 1984; 39 1985. Classificação: 595.7605 |
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